SrasV1, Main, Exploration, bibRecord, 004D76

Rapid and sensitive detection of severe acute respiratory syndrome coronavirus by rolling circle amplification

Identifieur interne : 004D76 ( Main/Exploration ); précédent : 004D75; suivant : 004D77

Rapid and sensitive detection of severe acute respiratory syndrome coronavirus by rolling circle amplification

Auteurs : BIN WANG [Australie] ; Simon J. Potter [Australie] ; YIGUANG LIN [Australie] ; Anthony L. Cunningham [Australie] ; Dominic E. Dwyer [Australie] ; YUELONG SU [République populaire de Chine] ; XUEJUN MA [République populaire de Chine] ; YUNDE HOU [République populaire de Chine] ; Nitin K. Saksena [Australie]

Source :

Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.

RBID : Pascal:05-0242073

Descripteurs français

Pascal (Inist)
- Coronavirus, Détection, Amplification, Microbiologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Amplification, Coronavirus, Detection, Microbiology, Severe acute respiratory syndrome.

Abstract

The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (n = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.

Affiliations:

Links toward previous steps (curation, corpus...)

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to stream PascalFrancis, to step Curation: 000313
to stream PascalFrancis, to step Checkpoint: 000592
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Le document en format XML

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<front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (n = 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.</div>
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Rapid and sensitive detection of severe acute respiratory syndrome coronavirus by rolling circle amplification

Rapid and sensitive detection of severe acute respiratory syndrome coronavirus by rolling circle amplification

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri